Cardiology journal · 2016
Enhancement of beta-catenin in cardiomyocytes suppresses survival protein expression but promotes apoptosis and fibrosis.
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Lin James C, Kuo Wei-Wen, Baskaran Rathinasamy, Chen Ming-Cheng, Ho Tsung-Jung, Chen Ray-Jade, Chen Ya-Fang, Vijaya Padma Viswanadha, Lay Ing-Shiow, Huang Chih-Yang
Аннотация
BACKGROUND: Beta-catenin has been implicated in cell-cell communication in a wide variety of developmental and physiological processes. Defective Wnt signaling could result in various cardiac and vascular abnormalities. Little is known regarding Wnt/frizzled pathway in cardiomyocyte apoptosis.
METHODS: In this study, the role of b-catenin in apoptosis was investigated in H9c2 cardiomyocytes and primary cardiomyocytes isolated in diabetic Wistar rats. The cardiomyocytes were transfected with porcine cytomegalovirus (pCMV)-b-catenin plasmid in order to overexpress b-catenin.
RESULTS: The transcription factor displayed a significant nuclear localization in Wistar rats with cardiac hypertension. Transfection of b-catenin plasmid induced apoptosis and reduced expression of survival pathway markers in cardiomyocytes in a dose-dependent manner. Furthermore, expression of fibrosis protein markers was upregulated by the overexpression.
CONCLUSIONS: Taken together, these results revealed that altered Wnt/b-catenin signaling might provoke heart failure. (Cardiol J 2017; 24, 2: 195-205).
METHODS: In this study, the role of b-catenin in apoptosis was investigated in H9c2 cardiomyocytes and primary cardiomyocytes isolated in diabetic Wistar rats. The cardiomyocytes were transfected with porcine cytomegalovirus (pCMV)-b-catenin plasmid in order to overexpress b-catenin.
RESULTS: The transcription factor displayed a significant nuclear localization in Wistar rats with cardiac hypertension. Transfection of b-catenin plasmid induced apoptosis and reduced expression of survival pathway markers in cardiomyocytes in a dose-dependent manner. Furthermore, expression of fibrosis protein markers was upregulated by the overexpression.
CONCLUSIONS: Taken together, these results revealed that altered Wnt/b-catenin signaling might provoke heart failure. (Cardiol J 2017; 24, 2: 195-205).
Предметные рубрики MeSH
AdultAgedAged, 80 and overAnimalsAnimals, NewbornApoptosisBlotting, WesternCardiomyopathiesCell SurvivalCells, CulturedDiabetes Mellitus, ExperimentalGene Expression Regulation, DevelopmentalHumansImmunohistochemistryMaleMiddle AgedMyocytes, CardiacRatsRats, WistarSignal TransductionSwinebeta Catenin