c-Fos protein expression in the anterior amygdaloid area and nc. accumbens in the hypoxic rat brain.

INTRODUCTION

By examining the production of c-Fos protein, we analyzed the response to the ischemic attack in different brain tissue, two of which are regions of the limbic system: the anterior amygdaloid area and nc. accumbens.

MATERIAL AND

METHODS

We used the model of rat brain ischemia--four-vessel occlusion, and Pulsinelli's method. The rats were treated in two ways, according to which they were divided into two groups: a total ischemia (ligation of four blood vessels, i.e. electrocauterization of the vertebral artery with bilateral ligation of the carotid artery--the so-called R-group rats), and transient ischemic attack (ligation of four blood vessels, i.e. electrocauterization of the vertebral artery, with mutual re-ligation of the carotid arteries in the form of transient ischemia--the so-called T-group rats, which can also be called "pre-conditioned group"). Both groups had their own control group.

CONCLUSION

We have concluded that parts of the brain with an important role for the survival have a strong expression of c-fos gene.