The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin.

Deltamethrin (Del), a widely utilized pyrethroid pesticide, exhibits significant risks to human health due to its persistent environmental residues. This study aims to develop an efficient sensing detector for rapid Del detection through aptamer-based recognition. A modified Capture-SELEX strategy successfully identified Del-1, a high-affinity DNA aptamer demonstrating specific binding to Del with a dissociation constant (Kd) of 82.90 ± 6.272 nM. Molecular docking analysis revealed strong intermolecular interactions between Del-1 and Del, exhibiting a favorable binding energy of -7.35 kcal·mol-1. Leveraging these findings, we constructed a colorimetric detector using gold nanoparticles (AuNPs) and poly dimethyl diallyl ammonium chloride (PDDA)-mediated aggregation modulation. The sensing detector employed dual detection parameters: (1) a characteristic color transition from red to blue and (2) a quantitative ∆A650/A520 ratio measurement. This optimized system achieved a detection limit of 54.57 ng·mL-1 with exceptional specificity against other competitive pesticides. Practical validation using spiked fruit samples (apples and pears) yielded satisfactory recoveries of 74-118%, demonstrating the sensor's reliability in real-sample analysis. The developed methodology presents a promising approach for the on-site monitoring of pyrethroid contaminants in agricultural products.