[Using DGGE to analyze bacteria community structure changes in subgingival plaque after periodontal initial therapy].

PURPOSE: To analyze bacteria community structure changes in subgingival plaque after initial therapy, and to provide experimental evidences for clinical decision.

METHODS

Six patients with chronic periodontitis were chosen. Subgingival plaque samples, as well as clinical indexes, were collected at baseline and six weeks after initial therapy. The generated two different 16S rDNA fragments of subgingival plaque samples were separated by denaturing gel, creating bands patterns representative of community structure. Subsequent cluster analysis was made.

RESULTS

The clinical indexes were improved significantly. The diversity of population of clinical subgingival samples was not significantly different between baseline and 6 weeks after initial therapy. Through cluster analysis, it was confirmed that same patient got similar subgingival plaque between baseline and 6 weeks after treatment.

CONCLUSIONS

Same patient tend to get similar subgingival plaque between baseline and after initial therapy. DGGE can detect the microbial composition of subgingival plaque.