Circulation research · 2015
Cardiac Myocyte KLF5 Regulates Ppara Expression and Cardiac Function.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Drosatos Konstantinos, Pollak Nina M, Pol Christine J, Ntziachristos Panagiotis, Willecke Florian, Valenti Mesele-Christina, Trent Chad M, Hu Yunying, Guo Shaodong, Aifantis Iannis, Goldberg Ira J
Аннотация
RATIONALE: Fatty acid oxidation is transcriptionally regulated by peroxisome proliferator-activated receptor (PPAR)α and under normal conditions accounts for 70% of cardiac ATP content. Reduced Ppara expression during sepsis and heart failure leads to reduced fatty acid oxidation and myocardial energy deficiency. Many of the transcriptional regulators of Ppara are unknown.
OBJECTIVE: To determine the role of Krüppel-like factor 5 (KLF5) in transcriptional regulation of Ppara.
METHODS AND RESULTS: We discovered that KLF5 activates Ppara gene expression via direct promoter binding. This is blocked in hearts of septic mice by c-Jun, which binds an overlapping site on the Ppara promoter and reduces transcription. We generated cardiac myocyte-specific Klf5 knockout mice that showed reduced expression of cardiac Ppara and its downstream fatty acid metabolism-related targets. These changes were associated with reduced cardiac fatty acid oxidation, ATP levels, increased triglyceride accumulation, and cardiac dysfunction. Diabetic mice showed parallel changes in cardiac Klf5 and Ppara expression levels.
CONCLUSIONS: Cardiac myocyte KLF5 is a transcriptional regulator of Ppara and cardiac energetics.
OBJECTIVE: To determine the role of Krüppel-like factor 5 (KLF5) in transcriptional regulation of Ppara.
METHODS AND RESULTS: We discovered that KLF5 activates Ppara gene expression via direct promoter binding. This is blocked in hearts of septic mice by c-Jun, which binds an overlapping site on the Ppara promoter and reduces transcription. We generated cardiac myocyte-specific Klf5 knockout mice that showed reduced expression of cardiac Ppara and its downstream fatty acid metabolism-related targets. These changes were associated with reduced cardiac fatty acid oxidation, ATP levels, increased triglyceride accumulation, and cardiac dysfunction. Diabetic mice showed parallel changes in cardiac Klf5 and Ppara expression levels.
CONCLUSIONS: Cardiac myocyte KLF5 is a transcriptional regulator of Ppara and cardiac energetics.
Предметные рубрики MeSH
AnimalsBinding SitesBinding, CompetitiveCardiomyopathy, DilatedCell LineDiabetes Mellitus, ExperimentalEnergy MetabolismFatty AcidsGenotypeKruppel-Like Transcription FactorsMice, Inbred C57BLMice, KnockoutMyocytes, CardiacOxidation-ReductionPPAR alphaPhenotypePromoter Regions, GeneticProtein BindingProto-Oncogene Proteins c-junSepsisSignal TransductionSodium-Glucose Transporter 2Sodium-Glucose Transporter 2 InhibitorsTime FactorsTranscription, GeneticTranscriptional ActivationTransfectionTriglyceridesUp-Regulation