Journal of dentistry · 2026
Interfacial and mechanical effects of methylglyoxal on etched dentin: An in vitro study.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Alzubaidi Wafa, Wang Bowen, Vaez Mina, Neshatian Mehrnoosh, Aguayo Sebastian, Thorpe Steven, Somogyi-Ganss Eszter, Bozec Laurent
Аннотация
OBJECTIVES: This study evaluated methylglyoxal (MGO) pretreatment of demineralized dentin with respect to (1) reinforcement of the dentin matrix, (2) resistance to enzymatic degradation, and (3) Stability of the resin-dentin bond under enzymatic challenge.
METHODS: Demineralized dentin was treated with 0.5, 1, or 3 M MGO and examined using attenuated total reflectance-Fourier transform infrared spectroscopy, collagenase degradation assays, and apparent elastic modulus before and after enzymatic exposure. Resin-dentin specimens were bonded using an etch-and-rinse adhesive, and microtensile bond strength (µTBS) was assessed after 24 h and following 30 days of collagenase aging.
RESULTS: MGO treatment increased carbohydrate-associated FTIR bands (1000-1180 cm⁻¹), consistent with glycation, with the largest effect at 3 M. Resistance to enzymatic degradation increased in a concentration-dependent manner, with the onset of degradation rising from 28 ± 2 min in controls to 35.9 ± 0.6 h in the 3 M group (p < 0.0001). The apparent elastic modulus increased from 1.07 ± 0.47 MPa (control) to 4.01 ± 0.97 MPa (3 M; p < 0.0001). After enzymatic challenge, the modulus of untreated dentin decreased to 0.34 ± 0.38 MPa (p = 0.013), whereas MGO-treated dentin retained significantly higher stiffness (p < 0.05). Immediate µTBS values did not differ among groups (p = 0.64). After 30 days of enzymatic aging, µTBS declined in controls (30.9 ± 6.0 to 17.3 ± 5.5 MPa; p = 0.027), while all MGO groups maintained significantly higher aged bond strength than controls (p < 0.01).
CONCLUSIONS: MGO pretreatment reinforced demineralized dentinal collagen and increased resistance to enzymatic degradation without affecting immediate bond strength, while preserving bond performance after proteolytic exposure.
CLINICAL SIGNIFICANCE: Reinforcing exposed dentin collagen through controlled glycation may help limit enzymatic degradation, which reduces the longevity of adhesive restorations.
METHODS: Demineralized dentin was treated with 0.5, 1, or 3 M MGO and examined using attenuated total reflectance-Fourier transform infrared spectroscopy, collagenase degradation assays, and apparent elastic modulus before and after enzymatic exposure. Resin-dentin specimens were bonded using an etch-and-rinse adhesive, and microtensile bond strength (µTBS) was assessed after 24 h and following 30 days of collagenase aging.
RESULTS: MGO treatment increased carbohydrate-associated FTIR bands (1000-1180 cm⁻¹), consistent with glycation, with the largest effect at 3 M. Resistance to enzymatic degradation increased in a concentration-dependent manner, with the onset of degradation rising from 28 ± 2 min in controls to 35.9 ± 0.6 h in the 3 M group (p < 0.0001). The apparent elastic modulus increased from 1.07 ± 0.47 MPa (control) to 4.01 ± 0.97 MPa (3 M; p < 0.0001). After enzymatic challenge, the modulus of untreated dentin decreased to 0.34 ± 0.38 MPa (p = 0.013), whereas MGO-treated dentin retained significantly higher stiffness (p < 0.05). Immediate µTBS values did not differ among groups (p = 0.64). After 30 days of enzymatic aging, µTBS declined in controls (30.9 ± 6.0 to 17.3 ± 5.5 MPa; p = 0.027), while all MGO groups maintained significantly higher aged bond strength than controls (p < 0.01).
CONCLUSIONS: MGO pretreatment reinforced demineralized dentinal collagen and increased resistance to enzymatic degradation without affecting immediate bond strength, while preserving bond performance after proteolytic exposure.
CLINICAL SIGNIFICANCE: Reinforcing exposed dentin collagen through controlled glycation may help limit enzymatic degradation, which reduces the longevity of adhesive restorations.