Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals · 2025
Involvement of PERK and ATF6 signal pathways in indicating unfolded protein response in patients with chronic lymphocytic leukaemia.
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Erkut Nergiz, Kestane Merve, Demir Selim, Mentese Ahmet, Balta Ozlen, Sonmez Mehmet
Аннотация
INTRODUCTION: PERK, ATF6, and IRE1α signalling pathways are unfolded protein response (UPR) signalling pathways. In this study, we evaluated the effect of PERK and ATF6 signalling pathways, in chronic lymphocytic leukaemia (CLL) patients.
METHODS: ELISA was performed on peripheral blood plasma samples from the CLL group and the control group for the markers eIF2AK3, GRP78, ATF6, CHOP, HIF-1α and caspase 3.
RESULTS: In this study, the levels of eIF2AK3, GRP78, ATF6 and CHOP were higher in the CLL group than in the control group (p = <0.001, p = <0.001, p = <0.001, p = <0.001, respectively). While no difference was observed between the CLL group and the control group in terms of HIF-1α level, caspase 3 level was higher in the CLL group (p = <0.001). There was a positive relationship between the level of HIF-1α and the levels of ATF6 and CHOP (r = 0.648, p = <0.001; r = 0.727, p = <0.001, respectively). Also, a positive correlation was observed between caspase 3 level and ATF6 level (r = 0.301, p = 0.030).
DISCUSSION: In this study, markers associated with PERK and ATF6 signalling pathways were higher in CLL patients.
METHODS: ELISA was performed on peripheral blood plasma samples from the CLL group and the control group for the markers eIF2AK3, GRP78, ATF6, CHOP, HIF-1α and caspase 3.
RESULTS: In this study, the levels of eIF2AK3, GRP78, ATF6 and CHOP were higher in the CLL group than in the control group (p = <0.001, p = <0.001, p = <0.001, p = <0.001, respectively). While no difference was observed between the CLL group and the control group in terms of HIF-1α level, caspase 3 level was higher in the CLL group (p = <0.001). There was a positive relationship between the level of HIF-1α and the levels of ATF6 and CHOP (r = 0.648, p = <0.001; r = 0.727, p = <0.001, respectively). Also, a positive correlation was observed between caspase 3 level and ATF6 level (r = 0.301, p = 0.030).
DISCUSSION: In this study, markers associated with PERK and ATF6 signalling pathways were higher in CLL patients.
Предметные рубрики MeSH
HumansActivating Transcription Factor 6eIF-2 KinaseLeukemia, Lymphocytic, Chronic, B-CellEndoplasmic Reticulum Chaperone BiPSignal TransductionMaleMiddle AgedFemaleUnfolded Protein ResponseHypoxia-Inducible Factor 1, alpha SubunitAgedTranscription Factor CHOPCaspase 3Case-Control StudiesHeat-Shock ProteinsAdultBiomarkers, Tumor