Journal of ethnopharmacology · 2025
From TCM "Shen-nourishing" and "Yang-strengthening" theory to blood-testis barrier reorganization, GuiLuBuShen attenuates age-related male reproductive dysfunction.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Jia Dan, Chen Di-Xin, Guo Qiu-Ping, Ou Hui-Yu, Liu Bo, Dai Wei-Ping, Peng Zi-Lun, Liu Yong-Jun, Wang Qi-Peng, Tan Qiu-Yi, Chen Wei, Liu Ju-Yan
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Traditional Chinese Medicine (TCM) provides a theoretical foundation for treating reproductive dysfunction via "Shen" system regulation. The classical formulation GuiLuBuShen pill (GLBS), recognized as a principal TCM therapy for male urogenital disorders, clinically enhances "Shen-Yang" nourishment in middle-aged and elderly males with genitourinary degeneration.
AIM OF THE STUDY: This study aims to elucidate the therapeutic efficacy and molecular mechanisms underlying GLBS in mitigating age-associated male genitourinary dysfunction, with particular focus on its regulatory effects on "Shen" deficiency-related pathophysiology during reproductive system senescence.
MATERIALS AND METHODS: In this study, 14-month-old Wistar rats were used to model natural male aging (vs. 6-week controls), and GLBS was administered at low (0.81 g/kg/d), medium (1.62 g/kg/d), and high (3.24 g/kg/d) doses for 8 weeks. The multimodal evaluation comprised physiological aging markers (body condition/fatigue recovery), reproductive competence (hormonal profiles/mating behavior/sperm parameters), organ integrity (morphometrics/urogenital histopathology) and molecular mechanisms (testicular transcriptomics & pathway validation).
RESULTS: GLBS treatment effectively attenuated age-related physiological decline, including weight loss, thermoregulatory dysfunction, and loco-motor impairment in open field test. Systemic anti physiological stress effects were demonstrated through reduced serum corticosterone, decreased organ degeneration and suppressed prostatic oxidative stress. GLBS restored reproductive function via reduced testicular oxidative damage, hormonal rebalancing, improved sperm motility/viability and attenuated seminiferous tubule degeneration with suppressed germ cell apoptosis. Mechanistic studies revealed that these effects were mechanistically linked to blood-testis barrier reinforcement and steroidogenic activation, collectively preserving spermatogenic homeostasis.
CONCLUSIONS: GLBS emerges as a multi-target therapeutic candidate for age-related urogenital disorders, uniquely combining systemic anti-aging effects with direct testicular rejuvenation. Its dual-action mechanism coordinates blood-testis barrier reinforcement through junctional remodeling with endocrine rebalancing, effectively preserving spermatogenic microenvironment homeostasis. The findings provide translational validation of traditional "Shen-nourishing" theory through contemporary molecular evidence, positioning GLBS as a promising intervention addressing both systemic senescence and organ-specific pathophysiology in male reproductive aging.
AIM OF THE STUDY: This study aims to elucidate the therapeutic efficacy and molecular mechanisms underlying GLBS in mitigating age-associated male genitourinary dysfunction, with particular focus on its regulatory effects on "Shen" deficiency-related pathophysiology during reproductive system senescence.
MATERIALS AND METHODS: In this study, 14-month-old Wistar rats were used to model natural male aging (vs. 6-week controls), and GLBS was administered at low (0.81 g/kg/d), medium (1.62 g/kg/d), and high (3.24 g/kg/d) doses for 8 weeks. The multimodal evaluation comprised physiological aging markers (body condition/fatigue recovery), reproductive competence (hormonal profiles/mating behavior/sperm parameters), organ integrity (morphometrics/urogenital histopathology) and molecular mechanisms (testicular transcriptomics & pathway validation).
RESULTS: GLBS treatment effectively attenuated age-related physiological decline, including weight loss, thermoregulatory dysfunction, and loco-motor impairment in open field test. Systemic anti physiological stress effects were demonstrated through reduced serum corticosterone, decreased organ degeneration and suppressed prostatic oxidative stress. GLBS restored reproductive function via reduced testicular oxidative damage, hormonal rebalancing, improved sperm motility/viability and attenuated seminiferous tubule degeneration with suppressed germ cell apoptosis. Mechanistic studies revealed that these effects were mechanistically linked to blood-testis barrier reinforcement and steroidogenic activation, collectively preserving spermatogenic homeostasis.
CONCLUSIONS: GLBS emerges as a multi-target therapeutic candidate for age-related urogenital disorders, uniquely combining systemic anti-aging effects with direct testicular rejuvenation. Its dual-action mechanism coordinates blood-testis barrier reinforcement through junctional remodeling with endocrine rebalancing, effectively preserving spermatogenic microenvironment homeostasis. The findings provide translational validation of traditional "Shen-nourishing" theory through contemporary molecular evidence, positioning GLBS as a promising intervention addressing both systemic senescence and organ-specific pathophysiology in male reproductive aging.