Molecular Changes Associated with Inflammation and Reproduction in Cadmium-Induced Testicular Toxicity: Mitigating Effect of Lactobacillus plantarum.

Cadmium (Cd) is a dangerous heavy metal that causes toxicity in humans and animals. Various protective agents are being investigated to ameliorate the toxic effect of Cd. Lactic acid bacteria are promising as a protective agent because of their ability to remove heavy metals from contaminated products. The aim of this study was to evaluate the effects of Lactobacillus plantarum on biomarkers associated with reproduction and inflammation in the testes of rats exposed to Cd. For the application, rats were randomly divided into four groups: Control group, Cd group, L. plantarum group, and combined group (Cd + L. plantarum). At the end of 21 days of oral administration, testicular tissues of the rats were removed and mRNA expression levels of genes associated with reproduction (Dazl, Amh, and Ddx4) and inflammation (Tnf-α, Il-6, Cox-2, Inos, Foxo1, Foxo3, and Nfkb) were determined. On the other hand, the amounts of NOS2/iNOS, 8-ohdg, and Tnfα were analyzed in the tissues. The mtDNA copy number was also investigated. Cd caused an increase in the expression level of inflammation-related genes, except Nfkb, and a decrease in the expression level of reproduction-related genes. It also increased the levels of TNF-α, iNOS, and 8-OHdG biomarkers and mtDNA copy number. However, L. plantarum treatment did not cause a significant change in these parameters. Moreover, L. plantarum exposure in combination with Cd attenuated the observed Cd-mediated molecular changes in testicular tissue. In conclusion, the findings suggest that L. plantarum administration may be beneficial against Cd-induced testicular toxicity and may be considered as a biological agent in the development of protective strategies against environmental pollutants with further studies.