Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography with light microscopy.

Here, we present a protocol combining quantitative micro-elastography (QME) with transmitted light (TL) and immunofluorescence (IF) microscopy to study ovarian elasticity and composition in situ. We describe steps for excising murine ovaries, acquiring QME scans, and fixing the tissue. Subsequently, the tissue is sectioned and imaged with TL microscopy to locate functional components, such as corpora lutea and follicles, and segment them within the QME data. Finally, IF is used to analyze cell and molecular composition. For complete details on the use and execution of this protocol, please refer to Jaeschke et al.1.