Journal of ethnopharmacology · 2025
LC-MS/MS profiling of Zanthoxylum piperitum (L.) DC. leaves cultivated in Egypt, isolation of its bioactive components, and interrelationships with anti-ulcerative activities: in vitro and in vivo approaches, molecular docking, and dynamics studies.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
El Tayeb Nermin S, Younis Nermin A, Mouneir Samar M, Ahmed Kawkab A, Al-Karmalawy Ahmed A, Alnajjar Radwan, Abdel-Monem Azza R, Fayek Nesrin M
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Zanthoxylum piperitum (L.) DC. cultivated in Egypt is a new cultivar of the known anti-inflammatory food spice Zanthoxylum piperitum (L.) DC.
AIM OF THE STUDY: This study aimed to investigate the new cultivar leaves phytochemically and biologically.
MATERIALS AND METHODS: UPLC-Triple TOF-MS/MS analysis examined the ethanolic extract's composition, and FRAP and ORAC assays evaluated its antioxidant activity. Major flavonoids were isolated and identified by spectroscopic techniques. The anti-inflammatory properties of the extract and isolated compounds were investigated in vitro. Then, by in vivo acetic acid-induced UC model on Sixty-three male Wistar rats at high and low doses compared to standard prednisolone. Evaluation involved macroscopic and microscopic assessment of rectal damage, cytokine measurement, molecular docking, and dynamic simulations to support the findings.
RESULTS: Extract of Zanthoxylum piperitum (L.) DC. Cultivated in Egypt demonstrated rich phenolic content and antioxidant action. Isoquercitrin and quercitrin were isolated and identified via spectroscopy. The in vitro anti-inflammatory tests showed that the extract inhibited COX1 and LOX significantly (p < 0.05). In the in vivo UC model, all treatments ameliorated the macroscopic and microscopic damage in the intestine of UC rats' and improved the biochemical markers in a dose-dependent manner, especially isoquercitrin at a high dose (40 mg/kg) showed the most significant results (p < 0.05) compared to the standard group. Molecular docking and dynamics studies supported these findings.
CONCLUSIONS: This indicates the potential of the extract of Zanthoxylum piperitum (L.) DC. cultivated in Egypt and its main constituents for the treatment of UC.
AIM OF THE STUDY: This study aimed to investigate the new cultivar leaves phytochemically and biologically.
MATERIALS AND METHODS: UPLC-Triple TOF-MS/MS analysis examined the ethanolic extract's composition, and FRAP and ORAC assays evaluated its antioxidant activity. Major flavonoids were isolated and identified by spectroscopic techniques. The anti-inflammatory properties of the extract and isolated compounds were investigated in vitro. Then, by in vivo acetic acid-induced UC model on Sixty-three male Wistar rats at high and low doses compared to standard prednisolone. Evaluation involved macroscopic and microscopic assessment of rectal damage, cytokine measurement, molecular docking, and dynamic simulations to support the findings.
RESULTS: Extract of Zanthoxylum piperitum (L.) DC. Cultivated in Egypt demonstrated rich phenolic content and antioxidant action. Isoquercitrin and quercitrin were isolated and identified via spectroscopy. The in vitro anti-inflammatory tests showed that the extract inhibited COX1 and LOX significantly (p < 0.05). In the in vivo UC model, all treatments ameliorated the macroscopic and microscopic damage in the intestine of UC rats' and improved the biochemical markers in a dose-dependent manner, especially isoquercitrin at a high dose (40 mg/kg) showed the most significant results (p < 0.05) compared to the standard group. Molecular docking and dynamics studies supported these findings.
CONCLUSIONS: This indicates the potential of the extract of Zanthoxylum piperitum (L.) DC. cultivated in Egypt and its main constituents for the treatment of UC.