Zhongguo shi yan xue ye xue za zhi · 2026
[Analysis of ABO Subtype Detection Methods and Hereditary Basis].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Wu Xin-Ming, Huo Ni, Gao Xu-Zhu, Zheng Dong
Аннотация
OBJECTIVE: Three methods were used to identify ABO blood group of discrepancies samples to determine the appropriate combination of identification methods and genetic mechanism.
METHODS: ABO blood group discrepancies samples were collected and identified with serological methods and molecular biological methods. The phenotype was determined with micro-column gel card and tube method. Molecular biological methods included the polymerase chain reaction with sequence-specific primers (PCR-SSP) for genotype determination, and genetic basis was determined by Sanger sequencing.
RESULTS: Among 28 samples, 15 ABO alleles were identified, including 5 A alleles, 5 B alleles, 2 O alleles, 1 B(A) allele and 2 CisAB alleles. The PCR-SSP genotyping results were inconsistent with sequencing genotyping results in 5 cases. Three novel base sequences were discovered by sequencing.
CONCLUSION: Combined serological methods and sequencing is helpful for precise ABO grouping, the combination can discover the subtype phenotype and genetic mechanism.
METHODS: ABO blood group discrepancies samples were collected and identified with serological methods and molecular biological methods. The phenotype was determined with micro-column gel card and tube method. Molecular biological methods included the polymerase chain reaction with sequence-specific primers (PCR-SSP) for genotype determination, and genetic basis was determined by Sanger sequencing.
RESULTS: Among 28 samples, 15 ABO alleles were identified, including 5 A alleles, 5 B alleles, 2 O alleles, 1 B(A) allele and 2 CisAB alleles. The PCR-SSP genotyping results were inconsistent with sequencing genotyping results in 5 cases. Three novel base sequences were discovered by sequencing.
CONCLUSION: Combined serological methods and sequencing is helpful for precise ABO grouping, the combination can discover the subtype phenotype and genetic mechanism.