[Analysis of ABO Subtype Detection Methods and Hereditary Basis].

OBJECTIVE

Three methods were used to identify ABO blood group of discrepancies samples to determine the appropriate combination of identification methods and genetic mechanism.

METHODS

ABO blood group discrepancies samples were collected and identified with serological methods and molecular biological methods. The phenotype was determined with micro-column gel card and tube method. Molecular biological methods included the polymerase chain reaction with sequence-specific primers (PCR-SSP) for genotype determination, and genetic basis was determined by Sanger sequencing.

RESULTS

Among 28 samples, 15 ABO alleles were identified, including 5 A alleles, 5 B alleles, 2 O alleles, 1 B(A) allele and 2 CisAB alleles. The PCR-SSP genotyping results were inconsistent with sequencing genotyping results in 5 cases. Three novel base sequences were discovered by sequencing.

CONCLUSION

Combined serological methods and sequencing is helpful for precise ABO grouping, the combination can discover the subtype phenotype and genetic mechanism.