The role of residual (veterinary) antibiotics in chemical exposome analysis: Current progress and future perspectives.

Humans are exposed to a complex mixture of environmental and food-related chemicals throughout their lifetime. Exposome research intends to explore the nongenetic, that is, environmental causes of chronic disease and their interactions comprehensively. Residual antibiotics can enter the human body through therapeutics, foods of animal origin, aquatic products, or drinking water. In the last decade, significant levels of residual antibiotics in human urine have been described, demonstrating frequent exposure throughout populations. To which extent they contribute to human health risks is debated. Human biomonitoring (HBM) aims to determine and quantify concentrations of xenobiotics in human specimens and provides the toolbox to monitor exposure to diverse chemical exposures. Due to their public health implications, priority-listed xenobiotics are routinely monitored in the European Union and other countries. However, antibiotics, an important class of (food-derived) xenobiotics, are still not systematically investigated for a better and more holistic understanding in the context of exposomics. This review provides a comprehensive summary of HBM research related to antibiotics, existing liquid chromatography-mass spectrometry (LC-MS)-based analytical methods, and potential health risks caused by unintended exposure. Incorporating antibiotics into the chemical exposome framework through routine HBM using multiclass analytical methods will provide a better understanding of the toxicological or pharmacological mixture effects and, ultimately, the chemical exposome.