[Screening of lipoprotein genes of Porphyromonas gingivalis in chronic periodontitis patients].

OBJECTIVE

To screen PG0717, PG0183 and PG2135 gene of Porphyromonas gingivalis (Pg) in subgingival plaque of the chronic periodontitis patients and periodontally healthy subjects and to investigate the relationship of these genes and periodontal clinical parameters.

METHODS

Forty-one chronic periodontitis patients and 76 periodontally healthy individuals were included. Clinical parameters were measured and recorded by a single examiner, which included bleeding on probing (BOP), attachment loss (AL), probing depth (PD), and tooth mobility (TM). Subgingival plaque samples were taken by sterile subgingival curette. The three genes, which were present in PgW83 but absent from PgATCC33277 were labeled with Cy5 and used as probes in DNA microarray hybridization. The PG0717, PG0183 and PG2135 gene in affected region, normal region and healthy individuals of Pg was examined.

RESULTS

The detecting rate of PG0717, PG0183 and PG2135 was 90% (18/20), 70% (14/20) and 70% (14/20) respectively in affected region, 60% (12/20), 45% (19/20) and 40% (8/20) respectively in normal region and 55% (11/20), 25% (5/20), 30% (6/20) in Pg of healthy individuals. The detecting rate of PG0717, PG0183 and PG2135 was significantly different between the affected region and the healthy individuals (P < 0.05).

CONCLUSIONS

The Pg with genes of PG0717, PG0183, PG2135 was more pathogenic.