The effects of combination of gefitinib and cisplatin on tongue squamous cell carcinoma cell lines.

OBJECTIVES

To study the inhibitory effect of combining the gefitinib with cisplatin on tongue squamous cell carcinoma cells.

MATERIALS AND

METHODS

Two cell lines were used, tca8113 which is a cisplatin-sensitive cell line, and tca8113/CDDP which is a cisplatin-resistant cell line established in Jiamusi University Laboratories. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to calculate the IC 50 values of both drugs using both cell lines. Detection of Ras, Raf and ERK1/2 was done by using western blot technique after exposure to different concentrations of gefitinib and cisplatin.

RESULTS

Tca8113/CDDP has shown more resistance to cisplatin than tca8113 and slightly more resistance to gefitinib than tca8113. Combination of the two drugs has shown to be more effective for both cell lines than when each alone even with lower concentrations.

CONCLUSIONS

Combination of gefitinib and cisplatin is a better choice than using each drug alone.