Golden Rice: A Quarter-Century of Innovation, Challenges, and the Promise of Better Nutrition.

Golden Rice was first introduced to the scientific community and the wider public 25 y ago and arguably remains the most widely recognized biofortified crop. Unlike most staples, the provitamin A carotenoid biosynthetic pathway is absent in the grain of all known rice cultivars. Scientists therefore relied on modern biotechnology to introduce 2 transgenes that enabled carotenoid production and accumulation, with 80%-90% as β-carotene. The second generation of Golden Rice, incorporating genes from maize and a common soil bacterium, can produce 20-30 μg of β-carotene per gram of edible rice. Although carotenoid levels decline in storage, they remain high enough to contribute meaningfully to vitamin A intake. After its initial development, Golden Rice technology was transferred to public sector breeding programs in low- and middle-income countries, prioritizing Bangladesh and the Philippines due to prevalent vitamin A deficiency, high per capita rice consumption, strong agricultural research institutes, and prior experience with transgenic crops. Both countries have developed locally adapted Golden Rice varieties matching conventional rice in agronomic performance, cultivation practices, and cost. However, regulatory hurdles have stalled their release-approval remains pending in Bangladesh, whereas legal challenges in the Philippines have at least temporarily halted further research and deployment. Although recent data indicate a decline in the prevalence of vitamin A deficiency in the Philippines, rural populations remain vulnerable, and Golden Rice has the potential to serve as a safety net to achieve adequate vitamin A intake.