Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology · 2026
p16 and MGMT Methylation in Chronic Traumatic Ulcers: A Plausible Link Between Chronic Mechanical Irritation and Oral Carcinogenesis?
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Lazos Jeronimo Pablo, Hernandez Rios Marcela, Astorga Jessica, Panico Rene Luis, Piemonte Eduardo David, Zárate Ana María
Аннотация
BACKGROUND: Chronic mechanical irritation (CMI) has been proposed as a risk factor for oral cancer. Epigenetic alterations, particularly methylation, are early events in carcinogenesis, and it has been proposed that they can be prompted by CMI. Thus, the aim of this study was to describe p16 and MGMT methylation in chronic traumatic ulcer (CTU).
METHODS: A case-control split-mouth study was performed. Two samples per individual (N = 27) were taken using exfoliative cytology using a split-mouth design, one from the CTU and the other from a contralateral site of clinically normal mucosa. DNA was extracted and bisulfite-treated, and specific sites at the promoter region of p16 and MGMT genes were amplified by qPCR using validated primers. Then, the PCR product was sequenced. The statistical analysis was performed by McNemar and the Chi-square test.
RESULTS: Patients had a mean age of 59.1 years. CTU showed higher methylation than control sites for both p16 (85% vs. 20%, p < 0.0001) and MGMT (80% vs. 24%, p < 0.0005).
CONCLUSION: Oral mucosa subjected to continuous exposure to CMI is associated with increased methylation of p16 and MGMT. Proper management of mechanical injury factors could be an important measure for OSCC prevention. In CMI, exfoliative cytology and the split-mouth design could be useful tools to study biomarkers. However, the role of CMI in oral carcinogenesis needs more evidence focusing on the biological phenomenon.
METHODS: A case-control split-mouth study was performed. Two samples per individual (N = 27) were taken using exfoliative cytology using a split-mouth design, one from the CTU and the other from a contralateral site of clinically normal mucosa. DNA was extracted and bisulfite-treated, and specific sites at the promoter region of p16 and MGMT genes were amplified by qPCR using validated primers. Then, the PCR product was sequenced. The statistical analysis was performed by McNemar and the Chi-square test.
RESULTS: Patients had a mean age of 59.1 years. CTU showed higher methylation than control sites for both p16 (85% vs. 20%, p < 0.0001) and MGMT (80% vs. 24%, p < 0.0005).
CONCLUSION: Oral mucosa subjected to continuous exposure to CMI is associated with increased methylation of p16 and MGMT. Proper management of mechanical injury factors could be an important measure for OSCC prevention. In CMI, exfoliative cytology and the split-mouth design could be useful tools to study biomarkers. However, the role of CMI in oral carcinogenesis needs more evidence focusing on the biological phenomenon.