Combinatorial chemistry & high throughput screening · 2026
The Regulatory Effects of Electroacupuncture on the Intestinal Flora of Mice with Ulcerative Colitis.
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Gao Xinyu, Xiao Enfan, Geng Shaohui, Jiang Haixu, Wang Hesong, Zhao Yuxin, Xie Jiaxin, Huang Guangrui, Jia Wenrui
Аннотация
INTRODUCTION: This study aimed to investigate the modulation of intestinal flora by electroacupuncture in a murine ulcerative colitis (UC) model, with a focus on analyzing microbial taxa and identifying key regulatory targets and pathways.
METHODS: A UC model was established in mice using 5% dextran sodium sulfate (DSS). Electroacupuncture was applied at bilateral "Shangjuxu" (electrostimulation) and "Tianshu" (manual acupuncture) points from days 5-9, while the mesalazine group received 0.5 g/kg/day via gavage. Disease activity index (DAI), colon length, and histopathology (hematoxylin-eosin staining) were evaluated. Intestinal flora composition was analyzed via 16S rDNA sequencing.
RESULTS: Electroacupuncture significantly reduced DAI scores on days 7 and 9 (P < 0.05; P < 0.01) compared to the model group, improved colon morphology, and reduced inflammation. Linear discriminant analysis and Wilcoxon tests revealed an increased abundance of Roseburia and elevated alpha diversity in the electroacupuncture group. Functional prediction demonstrated suppressed RNA transport and glycerophospholipid metabolism in the model group (P < 0.05), which were significantly enhanced post-electroacupuncture (P < 0.01).
DISCUSSION: Electroacupuncture restored beneficial taxa (e.g., Roseburia) and microbial diversity, suggesting gut homeostasis modulation. Enhanced lipid metabolism and RNA transport pathways may underlie its anti-inflammatory and mucosal repair effects.
CONCLUSION: Electroacupuncture alleviates UC by modulating the structure and function of intestinal flora, with Roseburia and associated metabolic pathways identified as key targets. These findings highlight the potential of electroacupuncture as a UC therapy.
METHODS: A UC model was established in mice using 5% dextran sodium sulfate (DSS). Electroacupuncture was applied at bilateral "Shangjuxu" (electrostimulation) and "Tianshu" (manual acupuncture) points from days 5-9, while the mesalazine group received 0.5 g/kg/day via gavage. Disease activity index (DAI), colon length, and histopathology (hematoxylin-eosin staining) were evaluated. Intestinal flora composition was analyzed via 16S rDNA sequencing.
RESULTS: Electroacupuncture significantly reduced DAI scores on days 7 and 9 (P < 0.05; P < 0.01) compared to the model group, improved colon morphology, and reduced inflammation. Linear discriminant analysis and Wilcoxon tests revealed an increased abundance of Roseburia and elevated alpha diversity in the electroacupuncture group. Functional prediction demonstrated suppressed RNA transport and glycerophospholipid metabolism in the model group (P < 0.05), which were significantly enhanced post-electroacupuncture (P < 0.01).
DISCUSSION: Electroacupuncture restored beneficial taxa (e.g., Roseburia) and microbial diversity, suggesting gut homeostasis modulation. Enhanced lipid metabolism and RNA transport pathways may underlie its anti-inflammatory and mucosal repair effects.
CONCLUSION: Electroacupuncture alleviates UC by modulating the structure and function of intestinal flora, with Roseburia and associated metabolic pathways identified as key targets. These findings highlight the potential of electroacupuncture as a UC therapy.