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Войти в исследования Shanghai kou qiang yi xue = Shanghai journal of stomatology · 2021
[Effect of PRRX1 on autophagy of salivary adenoid cystic carcinoma cells].
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Gao Jie, Jiang Ya-Ping, Ma Kang-Jie, Zhao Bao-Dong, Deng Jing
Аннотация
PURPOSE: To investigate the effect of paired related homeobox1 (PRRX1) on autophagy of salivary adenoidcystic carcinoma (SACC) cells.
METHODS: PRRX1-overexpressed lentiviral vectors and their negative control lentiviral vectors were used to transfect SACC cells. The transfection effect was detected by Western blot. Autophagosome was observed under transmission electron microscopy(TEM). The level of autophagy markers (LC3-II/Beclin1) was detected by immunofluorescence and Western blot. Statistical analysis was performed with SPSS 17.0 software package.
RESULTS: PRRX1 protein level increased, autophagosome number decreased, and autophagy marker level decreased in the PRRX1 overexpressed group, compared to the control (P<0.05).
CONCLUSIONS: PRRX1 inhibits the autophagy of SACC cells.
METHODS: PRRX1-overexpressed lentiviral vectors and their negative control lentiviral vectors were used to transfect SACC cells. The transfection effect was detected by Western blot. Autophagosome was observed under transmission electron microscopy(TEM). The level of autophagy markers (LC3-II/Beclin1) was detected by immunofluorescence and Western blot. Statistical analysis was performed with SPSS 17.0 software package.
RESULTS: PRRX1 protein level increased, autophagosome number decreased, and autophagy marker level decreased in the PRRX1 overexpressed group, compared to the control (P<0.05).
CONCLUSIONS: PRRX1 inhibits the autophagy of SACC cells.