A self-enhanced electrochemiluminescence aptasensor Zr-porphyrin modified with polyamidoamine for sensitive detection of lincomycin.

Exploring novel and sensitive analysis methods for monitoring lincomycin (Lin) residues is of great significance since overuse of it would cause a serious threat to public health. Herein, a Zr-porphyrin metal-organic frameworks (Zr-TCPP) with covalently modified polyamidoamine (PAMAM) dendrimers was synthesized as a novel intramolecular self-enhanced ECL reagent, which exhibited greatly improved ECL response due to the promotion of SO4•- generation and the shortening of the electron transfer distance. Graphene oxide modified with gold nanoparticles (Au@GO) was synthesized as the quencher for the ECL sensor construction based on the quenching strategy. The present aptasensor achieved a wide linear range of 1.0 × 10-14 - 5.0 × 10-9 g/mL and a low detection limit of 1.7 fg/mL, which was applied for the determination of Lin in different real samples with satisfactory results.