InterobServer AgreeMent in Pd-l1 evaLuatIoN on cytoloGical samples-SAMPLING project: A multi-institutional, international study.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
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Acanfora Gennaro, Iaccarino Antonino, Cerbelli Bruna, Di Cristofano Claudio, Bellevicine Claudio, Barberis Massimo, Bonoldi Emanuela, Bubendorf Lukas, Calaminus Andreas, Campione Severo, Canberk Sule, Cavazza Alberto, Cazzaniga Giorgio, Chijioke Obinna, Clery Eduardo, Eccher Albino, Engels Marianne, Fiorentino Vincenzo, Graziano Paolo, Kern Izidor, Kholova Ivana, Laatta Jari, Labiano Tania, Leopizzi Martina, Lozano Maria D, Luis Rita, Maffei Elisabetta, Marando Alessandro, Martini Maurizio, Merenda Elisabetta, Montella Marco, Morales Allan Argueta, Nishino Michiya, Pagni Fabio, Hofman Paul, Pernazza Angelina, Roy-Chowdhuri Sinchita, Saieg Mauro, Savic Prince Spasenija, Siddiqui Momin T, Stoos-Veic Tajana, Strojan Fležar Margareta, Sundov Dinka, VanderLaan Paul, Vrdoljak-Mozetič Danijela, Zeppa Pio, Troncone Giancarlo, Vigliar Elena
Аннотация
METHODS: A total of 65 cell blocks (CB) diagnosed as NSCLC were retrospectively collected and selected for TMA preparation. Hematoxylin-eosin and PD-L1 stained slides were digitized and uploaded on a free web sharing platform. Participants were asked to provide PD-L1 assessment by using the clinically relevant cutoff of tumor proportion score (TPS) (<1%; 1%-49%; >50%). Interobserver agreement was calculated using Fleiss's κ.
RESULTS: Of 65 CBs, 11 were deemed not suitable; therefore, an overall number of 54 cores were used for the preparation of four TMAs. A total of 1674 evaluations were provided by 31 cytopathologists from 21 different institutions in nine countries. The statistical analysis showed a moderate overall agreement (κ = 0.49). The highest agreement was achieved in the TPS >50% category (κ = 0.57); moderate agreement was observed in TPS <1% category (κ = 0.51) and the lowest κ value was obtained for TPS 1%-49% category (k = 0.32).
CONCLUSIONS: The overall moderate agreement observed showed that there is still room for improvement in inter-pathologist agreement for PD-L1 evaluation on cytological samples, highlighting the need for standardization in sample preparation, focused training in PD-L1 evaluation on cytological material, and the integration of machine learning tools to improve interobserver consistency.