Prostaglandins & other lipid mediators · 2022
Rosa damascena Miller essential oil relaxes rat trachea via K<sub>V</sub> channels, K<sub>ATP</sub> channels, and BK<sub>Ca</sub> channels.
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Demirel Sadettin
Аннотация
AIM/OBJECTIVE: This study aimed to investigate the effects of Rosa damascena Miller essential oil on rat tracheal smooth muscle contractility and the hypothesis that voltage-gated potassium (K<sub>V</sub>) channels, ATP-sensitive potassium (K<sub>ATP</sub>) channels, and large-conductance calcium-activated potassium (BK<sub>Ca</sub>) channels may have roles in these effects.
METHODS: Isometric contraction-relaxation responses of tracheal rings were measured with an isolated tissue bath model. The steady contraction was induced with both 10<sup>-5</sup> M ACh and 60 mM KCl, and then the concentration-dependent responses of rose oil (0.1-100 µg/mL) were examined. The time-matched control (double distilled water) group was also formed. To evaluate the role of K<sub>V</sub>, K<sub>ATP</sub>, and BK<sub>Ca</sub> channels, tracheal rings were incubated with 4-AP (K<sub>V</sub> channel blocker), glibenclamide (K<sub>ATP</sub> channel blocker), TEA (BK<sub>Ca</sub> channel blocker), and iberiotoxin (selective BK<sub>Ca</sub> channel blocker). Also, a vehicle control group was formed for dimethyl sulfoxide (DMSO).
RESULTS: Rose oil exerted the relaxant effects in tracheal rings pre-contracted with both ACh and KCl at concentrations of 1, 10, and 100 µg/mL (p < 0.05). Besides, K<sub>V</sub> channel blocker 4-AP, K<sub>ATP</sub> channel blocker glibenclamide, and BK<sub>Ca</sub> channel blockers TEA and iberiotoxin incubations significantly inhibited the rose oil-induced relaxation responses (p < 0.05). However, incubation of tissues with DMSO, glibenclamide solvent, for 10 min did not cause a significant change in the relaxation responses to rose oil (p > 0.05).
CONCLUSIONS: In conclusion, the first physiological findings were obtained regarding the functional relaxant effects of rose essential oil in rat trachea. The findings showed that rose oil induces bronchorelaxation in a concentration-dependent manner. Besides, this study is the first to report that rose oil-mediated bronchodilation responses are associated with the activity of K<sub>V</sub>, K<sub>ATP</sub>, and BK<sub>Ca</sub> channels. These results suggest that rose oil might be a useful agent in the treatment of abnormal bronchoconstriction-related diseases such as asthma and chronic obstructive pulmonary disease.
METHODS: Isometric contraction-relaxation responses of tracheal rings were measured with an isolated tissue bath model. The steady contraction was induced with both 10<sup>-5</sup> M ACh and 60 mM KCl, and then the concentration-dependent responses of rose oil (0.1-100 µg/mL) were examined. The time-matched control (double distilled water) group was also formed. To evaluate the role of K<sub>V</sub>, K<sub>ATP</sub>, and BK<sub>Ca</sub> channels, tracheal rings were incubated with 4-AP (K<sub>V</sub> channel blocker), glibenclamide (K<sub>ATP</sub> channel blocker), TEA (BK<sub>Ca</sub> channel blocker), and iberiotoxin (selective BK<sub>Ca</sub> channel blocker). Also, a vehicle control group was formed for dimethyl sulfoxide (DMSO).
RESULTS: Rose oil exerted the relaxant effects in tracheal rings pre-contracted with both ACh and KCl at concentrations of 1, 10, and 100 µg/mL (p < 0.05). Besides, K<sub>V</sub> channel blocker 4-AP, K<sub>ATP</sub> channel blocker glibenclamide, and BK<sub>Ca</sub> channel blockers TEA and iberiotoxin incubations significantly inhibited the rose oil-induced relaxation responses (p < 0.05). However, incubation of tissues with DMSO, glibenclamide solvent, for 10 min did not cause a significant change in the relaxation responses to rose oil (p > 0.05).
CONCLUSIONS: In conclusion, the first physiological findings were obtained regarding the functional relaxant effects of rose essential oil in rat trachea. The findings showed that rose oil induces bronchorelaxation in a concentration-dependent manner. Besides, this study is the first to report that rose oil-mediated bronchodilation responses are associated with the activity of K<sub>V</sub>, K<sub>ATP</sub>, and BK<sub>Ca</sub> channels. These results suggest that rose oil might be a useful agent in the treatment of abnormal bronchoconstriction-related diseases such as asthma and chronic obstructive pulmonary disease.