Psoriasis Is Accompanied by Low Serum Levels of MG-H1, GOLD and MOLD: LC-Orbitrap-MS/MS Analysis of Chosen Glycation Products.

Glycation is a type of post-translational protein modification that affects antigen self-recognition, cell signaling, the proteasomal degradation of proteins, protein solubility, and the mechanical properties of tissues. Some glycation products are able to cross-link proteins. Serum concentrations of the cross-linking GOLD (glyoxal lysine dimer) and MOLD (methylglyoxal lysine dimer), and the non-cross-linking MG-H1 (methylglyoxal-derived hydroimidazolone isomer 1) in patients with psoriasis (n = 63) and in healthy controls (n = 35) were examined using the LC-Orbitrap-MS/MS (liquid chromatography-Orbitrap tandem mass spectrometry) technique. The following indices were assessed in the patients: BSA (body surface area), PASI (psoriasis area and severity index), and DLQI (Dermatology Life Quality Index). Serum concentrations of GOLD, MOLD, and MG-H1 were found to be significantly lower in psoriasis patients compared with healthy individuals. The concentrations of GOLD, MOLD, and MG-H1 did not correlate with the indices of disease activity and severity. These results may reflect the complexity of metabolic dysregulation accompanying immune-mediated inflammatory diseases such as psoriasis.