[Contribution of Parodium gel in an experimental model of human gingival inflammation].

INTRODUCTION

Our aim was to evaluate the effect of a gingival gel containing chlorhexidine and Rheum Palmatum extract on gingival fragments stimulated by SP (substance P) and lipopolysaccharides (LPS).

MATERIALS AND

METHODS

Gingival fragments were maintained in survival for 3 days at 37 degrees C. To induce inflammation, SP and LPS were applied to the culture medium in contact with the corium. The gingival gel was applied on epithelium. Histological analysis was then performed on hematoxylin and eosin stained slides. Edema was evaluated with semi-quantitative scores. Vasodilation was studied by counting the percent of dilated vessels according to scores and the surface of these dilated vessels by morphometrical image analysis. An inflammatory cytokine, IL8, was measured in culture supernatants. Immunohistochemical expression of metalloproteinase type 9 (MMP9) implicated in inflammatory processes, was also studied (% of positive cells).

RESULTS

Edema, vasodilation and IL8 were significantly increased after application of SP and LPS. Application of gingival gel showed a significant decrease of these parameters. A significant decrease of MMP9 on fibroblasts and mononuclear cells was observed after use of gingival gel.