An ex vivo human placental explant model for controlled exposure studies.

The human placental explant model preserves the placenta's natural structure, enabling studies on whole-tissue response to exposures or growth conditions. It outlines procedures for placental collection, isolation, and culturing, followed by exposure to conditions of interest. Additionally, it details sample collection of explant tissue and culture supernatant after exposure for downstream assessments of placental function. This protocol provides a framework for evaluating ex vivo responses of human placental tissue. For complete details on the use and execution of this protocol, please refer to Gonzalez et al.1.