JCI insight · 2024
PFKFB3 controls acinar IP3R-mediated Ca2+ overload to regulate acute pancreatitis severity.
СтатьяПоддержка исследования: вне правительства СШАПоддержка NIH: внешняя программаНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Zhang Tan, Chen Shengchuan, Li Liang, Jin Yuepeng, Liu Siying, Liu Zhu, Shi Fengyu, Xie Lifen, Guo Panpan, Cannon Andrew C, Ergashev Akmal, Yao Haiping, Huang Chaohao, Zhang Baofu, Wu Lijun, Sun Hongwei, Chen Siming, Shan Yunfeng, Yu Zhengping, Tolosa Ezequiel J, Liu Jianghuai, Fernandez-Zapico Martin E, Ma Feng, Chen Gang
Аннотация
Acute pancreatitis (AP) is among the most common hospital gastrointestinal diagnoses; understanding the mechanisms underlying the severity of AP is critical for development of new treatment options for this disease. Here, we evaluate the biological function of phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) in AP pathogenesis in 2 independent genetically engineered mouse models of AP. PFKFB3 was elevated in AP and severe AP (SAP), and KO of Pfkfb3 abrogated the severity of alcoholic SAP (FAEE-SAP). Using a combination of genetic, pharmacological, and molecular studies, we defined the interaction of PFKFB3 with inositol 1,4,5-trisphosphate receptor (IP3R) as a key event mediating this phenomenon. Further analysis demonstrated that the interaction between PFKFB3 and IP3R promotes FAEE-SAP severity by altering intracellular calcium homeostasis in acinar cells. Together, our results support a PFKFB3-driven mechanism controlling AP pathobiology and define this enzyme as a therapeutic target to ameliorate the severity of this condition.