Asian Pacific journal of tropical medicine · 2014
Effect of bone marrow mesenchymal stem cells on the Smad expression of hepatic fibrosis rats.
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Wang Zhen-Chang, Yang Shan, Huang Jing-Jing, Chen Song-Lin, Li Quan-Qiang, Li Yuan
Аннотация
OBJECTIVE: To investigate the impact of bone marrow mesenchymal stem cells on Smad expression of hepatic fibrosis rats.
METHODS: A total of 48 adult female SD rats were randomly divided into three groups, normal control group (n=10), observation group (n=19) with liver fibrosis model rats injected with BMSCs cells; model group (n=19), with liver fibrosis model rats injected with physiological saline. Serum index, TGF-β1 and Smad expression were detected.
RESULTS: Type III procollagen, IV collagen, hyaluronic acid, laminin levels of observation group were significantly lower than those of model group (P<0.05). The content and expression of TGF-β1 in serum and liver tissue of observation group were significantly lower than those of model group(P<0.05). Compared with normal control group, the Smad3, Smad4 mRNA and protein expression of model group were significantly increased, the Smad7 mRNA and protein expression were significantly reduced (P<0.05). Compared with model group, Smad3, Smad4 mRNA and protein expression of observation group were significantly reduced, and Smad7 mRNA expression were significantly increased (P<0.05).
CONCLUSIONS: BMSCs can regulate Smad expression to some extent, and reduce the degree of liver fibrosis.
METHODS: A total of 48 adult female SD rats were randomly divided into three groups, normal control group (n=10), observation group (n=19) with liver fibrosis model rats injected with BMSCs cells; model group (n=19), with liver fibrosis model rats injected with physiological saline. Serum index, TGF-β1 and Smad expression were detected.
RESULTS: Type III procollagen, IV collagen, hyaluronic acid, laminin levels of observation group were significantly lower than those of model group (P<0.05). The content and expression of TGF-β1 in serum and liver tissue of observation group were significantly lower than those of model group(P<0.05). Compared with normal control group, the Smad3, Smad4 mRNA and protein expression of model group were significantly increased, the Smad7 mRNA and protein expression were significantly reduced (P<0.05). Compared with model group, Smad3, Smad4 mRNA and protein expression of observation group were significantly reduced, and Smad7 mRNA expression were significantly increased (P<0.05).
CONCLUSIONS: BMSCs can regulate Smad expression to some extent, and reduce the degree of liver fibrosis.