Lnk/Sh2b3 regulates initiation and severity of autoimmune insulitis and contributes to diabetes risk.

The Lnk/Sh2b3 adaptor protein functions as a regulatory molecule for cytokine signaling in lymphohematopoiesis. A missense variant of the LNK/SH2B3 gene is reportedly a risk variant common to several autoimmune diseases, including type 1 diabetes (T1D). However, roles of Lnk in T1D development remain elusive. We found that Lnk -/- mice showed increased susceptibility to diabetes following treatment with fairly low doses of streptozotocin, manifested by hyperglycemia and insulitis accompanied by accumulation of CD8+ T-cells and loss of pancreatic β cells. The high susceptibility of Lnk -/- mice to islet damage was abolished in crosses with Rag2 -/- mice lacking lymphocytes or MyD88 -/- mice carrying various defects in activation of innate immune cells. In Lnk -/- mice pancreata, dendritic cell (DC) fractions were altered and showed augmented expression of CD40 and IL-27. Treatment with anti-CD40L or anti-GM-CSF antibodies suppressed β cell damage and prevented diabetes. Thus, Lnk regulates T-cell priming and expansion via GM-CSF- and possibly IL-27-dependent activation of pancreatic DCs after islet damage.