American journal of reproductive immunology (New York, N.Y. : 1989) · 2005
Regulation and signal transduction of toll-like receptors in human chorioncarcinoma cell lines.
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Klaffenbach Daniela, Rascher Wolfgang, Röllinghoff Martin, Dötsch Jörg, Meissner Udo, Schnare Markus
Аннотация
PROBLEM: Toll-like receptors (TLRs) are expressed on placental cells. The aim of this study is to analyze signaling components activated in placenta cells after TLR ligand engagement.
METHODS OF STUDY: In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase chain reaction as well as by fluorescence-activated cell sorter analysis. Activation of NF-kappaB was determined in a reporter assay system and the activation of the mitogen-activated protein kinase signaling pathways by immunoblot analysis.
RESULTS: Both lipopolysaccharide (LPS) and DNA oligonucleotides containing unmethylated CpG motifs (CpG) induced the enhanced expression of TLR2 mRNA as well as a TLR2 surface protein expression. Functionally, incubation of JAR cells with microbial stimuli such as LPS activated NF-kappaB, as well as the phosphorylation of ERK1/2 and p38 MAP kinases and secretion of interleukin-8.
CONCLUSION: The functional expression of TLRs on placental cells may play an important role in the initiation of an immune response in the developing fetus.
METHODS OF STUDY: In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase chain reaction as well as by fluorescence-activated cell sorter analysis. Activation of NF-kappaB was determined in a reporter assay system and the activation of the mitogen-activated protein kinase signaling pathways by immunoblot analysis.
RESULTS: Both lipopolysaccharide (LPS) and DNA oligonucleotides containing unmethylated CpG motifs (CpG) induced the enhanced expression of TLR2 mRNA as well as a TLR2 surface protein expression. Functionally, incubation of JAR cells with microbial stimuli such as LPS activated NF-kappaB, as well as the phosphorylation of ERK1/2 and p38 MAP kinases and secretion of interleukin-8.
CONCLUSION: The functional expression of TLRs on placental cells may play an important role in the initiation of an immune response in the developing fetus.
Предметные рубрики MeSH
Cell Line, TumorChoriocarcinomaFemaleGene Expression RegulationHumansInterleukin-8LipopolysaccharidesMembrane GlycoproteinsMitogen-Activated Protein KinasesNF-kappa BOligonucleotidesPlacentaPregnancyRNA, MessengerReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionSignal TransductionToll-Like Receptor 2Toll-Like ReceptorsTrophoblasts