Current eye research · 1997
Distribution pattern of nervous tissue and peptidergic nerve fibers in accessory lacrimal glands.
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Seifert P, Stuppi S, Spitznas M
Аннотация
PURPOSE: Demonstration of the distribution pattern of nervous tissue and the occurrence of neuropept human accessory lacrimal glands by means of immunohistochemical methods.
METHODS: Paraffin sections of tissue samples were incubated with antisera against protein gene product (PGP), S-100 protein (S-100), calcitonin gene-related peptide (CGRP) and substance P (SP). The immunoreactions were visualized using both species-specific secondary antibodies and a streptavidin-biotin-peroxidase complex (ABC method).
RESULTS: PGP- and S-100-immunoreactive nerve fibers were distributed in the entire intertubular stroma. In addition, myoepithelial cells, a few clusters of tubulus cells, groups of wandering cells, and scattered cells of the excretory duct system were S-100-immunoreactive. CGRP- and SP-immunoreactivity was found in a few nerve fibers in the intertubular glandular stroma with an association to secretory tubules, blood vessels, intralobular ducts and excretory duct.
CONCLUSIONS: These observations are in agreement with findings in the main lacrimal gland.
METHODS: Paraffin sections of tissue samples were incubated with antisera against protein gene product (PGP), S-100 protein (S-100), calcitonin gene-related peptide (CGRP) and substance P (SP). The immunoreactions were visualized using both species-specific secondary antibodies and a streptavidin-biotin-peroxidase complex (ABC method).
RESULTS: PGP- and S-100-immunoreactive nerve fibers were distributed in the entire intertubular stroma. In addition, myoepithelial cells, a few clusters of tubulus cells, groups of wandering cells, and scattered cells of the excretory duct system were S-100-immunoreactive. CGRP- and SP-immunoreactivity was found in a few nerve fibers in the intertubular glandular stroma with an association to secretory tubules, blood vessels, intralobular ducts and excretory duct.
CONCLUSIONS: These observations are in agreement with findings in the main lacrimal gland.