Molecular Docking and Single-Cell RNA-Seq Analysis Identify PTGS2 as a Key Target of Osthole in the Oral Squamous Cell Carcinoma Microenvironment.

INTRODUCTION

This study investigates the therapeutic effects of Osthole and elucidates its mechanisms in oral squamous cell carcinoma (OSCC).

MATERIALS AND

METHODS

Differential expression analysis was performed, followed by nomogram construction, gene set enrichment analysis, and immune infiltration analysis. Molecular docking was conducted to evaluate binding interactions, and single-cell analysis was performed.

RESULTS

PTGS2 was identified as a key candidate capable of binding with Osthole. Immune infiltration analysis revealed elevated levels of activated inflammatory cells in OSCC. Single-cell analysis further showed high PTGS2 expression in macrophages and mast cells.

DISCUSSION: This study demonstrates PTGS2's involvement in OSCC, highlighting its potential as both a biomarker and a therapeutic target.

CONCLUSION

Osthole can modulate OSCC by targeting PTGS2, providing a theoretical basis for OSCC management.