Medical science monitor : international medical journal of experimental and clinical research · 2012
Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Kim Jae Ho, Lee Sang Kwang, Yoo Yong Cheol, Park Nam Hyun, Park Dan Bi, Yoo Jong Shin, An Hyun Joo, Park Young Mok, Cho Kyung Gi
Аннотация
BACKGROUND: To identify meningioma-specific proteins, cerebrospinal fluid (CSF) from 4 patients with a meningioma and 4 patients with a non-brain tumorous lesion were analyzed.
MATERIAL/METHODS: Two-dimensional electrophoresis and electrospray quadrupole time-of-flight tandem mass spectrometry analyses revealed 10 unique spots, containing 11 independent proteins (spot #2 and #4 each contained 2 proteins and spot #3 was not identified) were evident in CSF associated with human meningioma: serum albumin precursor (3 different isoforms), Apolipoprotein E (Apo E), Apolipoprotein J precursor (Apo J), Transthyretin precursor (TTR), Prostaglandin D2 synthase 21 kDa (PTGDS), proapolipoprotein, Chain D hemoglobin Ypsilanti, alpha-1-antitrypsin (AAT), and beta-2-microglobulin precursor (β2M).
RESULTS: The contents of Apo E, Apo J and AAT were increased, while PTGDS, TTR and β2M were decreased.
CONCLUSIONS: The results observed by 2-dimensional electrophoresis were verified by Western blot analysis. The unique proteins may represent possible candidate biomarkers of meningioma.
MATERIAL/METHODS: Two-dimensional electrophoresis and electrospray quadrupole time-of-flight tandem mass spectrometry analyses revealed 10 unique spots, containing 11 independent proteins (spot #2 and #4 each contained 2 proteins and spot #3 was not identified) were evident in CSF associated with human meningioma: serum albumin precursor (3 different isoforms), Apolipoprotein E (Apo E), Apolipoprotein J precursor (Apo J), Transthyretin precursor (TTR), Prostaglandin D2 synthase 21 kDa (PTGDS), proapolipoprotein, Chain D hemoglobin Ypsilanti, alpha-1-antitrypsin (AAT), and beta-2-microglobulin precursor (β2M).
RESULTS: The contents of Apo E, Apo J and AAT were increased, while PTGDS, TTR and β2M were decreased.
CONCLUSIONS: The results observed by 2-dimensional electrophoresis were verified by Western blot analysis. The unique proteins may represent possible candidate biomarkers of meningioma.
Предметные рубрики MeSH
AdultAgedAmino Acid SequenceBiomarkers, TumorBlotting, WesternElectrophoresis, Gel, Two-DimensionalFemaleHumansImmunohistochemistryMaleMeningeal NeoplasmsMeningiomaMiddle AgedMolecular Sequence AnnotationMolecular Sequence DataNeoplasm ProteinsProteomeProteomicsReproducibility of ResultsSpectrometry, Mass, Electrospray Ionization