Медицинская библиотека · Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials · 2013

[Effects of plumbagin on expression of TNF-alpha and PDGF-BB in human hepatic stellate cells activated by leptin].

Liu Xue-Mei, Zhao Tie-Jian, Peng Yue, Duan Xue-Lin, Wei Yan-Fei

СтатьяПоддержка исследования: вне правительства СШАПеревод пока не подготовлен

Аннотация

OBJECTIVE

To investigate the effect of plumbagin on the expression of TNF-alpha and PDGF-BB in human hepatic stellate cells (HSC-LX2) activated by Leptin.

METHODS

HSC-LX2 were cultured in vitro and stimulated by Leptin for 24 hours then treated with different concentrations of plumbagin for 24 hours, the expressions of TNF-alpha mRNA and PDGF-BB mRNA were determined by Realtime quantitative PCR, the protein expressions of TNF-alpha and PDGF-BB were determined-by Western blotting.

RESULTS

The expressions of TNF-alpha mRNA and PDGF-BB mRNA of treatment groups were significantly reduced, especially in high dose group (P < 0.01), and Western blotting analyses revealed similar trends in protein expression.

CONCLUSION

Plumbagin may prevent the formation of hepatic fibrosis and its mechanism may be related to decreasing the level of mRNA of TNF-alpha and PDGF-BB and the protein of PDGF-BB.

Для цитирования: Liu Xue-Mei, Zhao Tie-Jian, Peng Yue, Duan Xue-Lin, Wei Yan-Fei. [Effects of plumbagin on expression of TNF-alpha and PDGF-BB in human hepatic stellate cells activated by leptin].. Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials. 2013.

Предметные рубрики MeSH

Becaplermin Cells, Cultured Gene Expression Regulation Hepatic Stellate Cells Humans Leptin Liver Cirrhosis Naphthoquinones Plumbaginaceae Polymerase Chain Reaction Proto-Oncogene Proteins c-sis RNA, Messenger Tumor Necrosis Factor-alpha

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