Intermediate Conductance Calcium-Dependent Potassium Channel (KCa3.1) Interacting Proteins Using Turboid-Based Proximity Labeling Technology: Insights Into Interactome and Related Signaling Pathways in Pancreatic Tumors.

KCa3.1 ion channel is a calcium-activated potassium channel expressed in various tissues, showing dual localization to the plasma membrane and to mitochondria. This channel is highly expressed in numerous cancers and has been implicated in the regulation of proliferation and migration. The molecular details of the signaling pathways linked to regulation exerted by KCa3.1 in cancer cells are, however, not fully elucidated yet. Therefore, we determined the interactome of KCa3.1 using proximity labeling in intact KPC pancreatic cancer cells that mirror the aggressive metastatic behavior of human pancreatic cancer. The results highlight several novel interactors, including those residing in intracellular membranes. The KCa3.1 channel proxisome and related pathways are discussed in light of our current knowledge about KCa3.1 and pancreatic cancer, available in public databases.