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Войти в исследования Anticancer research · 2018
The Potential of Exosomes Derived from Chronic Myelogenous Leukaemia Cells as a Biomarker.
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Kang Ka-Won, Jung Jik-Han, Hur Woojune, Park Jaena, Shin Hyunku, Choi Byeonghyeon, Jeong Hyesun, Kim Dae Sik, Yu Eun Sang, Lee Se Ryeon, Sung Hwa Jung, Kim Seok Jin, Choi Chul Won, Kim Hyun Koo, Hong Sunghoi, Park Ji-Ho, Choi Yeonho, Park Yong, Kim Byung Soo
Аннотация
BACKGROUND/AIM: Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities.
MATERIALS AND METHODS: Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick™ exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript.
RESULTS: Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This ~250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript.
CONCLUSION: CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.
MATERIALS AND METHODS: Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick™ exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript.
RESULTS: Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This ~250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript.
CONCLUSION: CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.