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DOI 10.21873/anticanres.12679

Медицинская библиотека · Anticancer research · 2018

The Potential of Exosomes Derived from Chronic Myelogenous Leukaemia Cells as a Biomarker.

Kang Ka-Won, Jung Jik-Han, Hur Woojune, Park Jaena, Shin Hyunku, Choi Byeonghyeon, Jeong Hyesun, Kim Dae Sik, Yu Eun Sang, Lee Se Ryeon, Sung Hwa Jung, Kim Seok Jin, Choi Chul Won, Kim Hyun Koo, Hong Sunghoi, Park Ji-Ho, Choi Yeonho, Park Yong, Kim Byung Soo

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Аннотация

BACKGROUND/AIM: Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities.

MATERIALS AND

METHODS

Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick™ exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript.

RESULTS

Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This ~250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript.

CONCLUSION

CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.

Для цитирования: Kang Ka-Won, Jung Jik-Han, Hur Woojune, Park Jaena, Shin Hyunku, Choi Byeonghyeon, Jeong Hyesun, Kim Dae Sik, Yu Eun Sang, Lee Se Ryeon, Sung Hwa Jung, Kim Seok Jin, Choi Chul Won, Kim Hyun Koo, Hong Sunghoi, Park Ji-Ho, Choi Yeonho, Park Yong, Kim Byung Soo. The Potential of Exosomes Derived from Chronic Myelogenous Leukaemia Cells as a Biomarker.. Anticancer research. 2018. DOI: 10.21873/anticanres.12679.

Предметные рубрики MeSH

Biomarkers, TumorBlotting, WesternCell Line, TumorExosomesGenes, ablHumansLeukemia, Myelogenous, Chronic, BCR-ABL PositiveMicroscopy, Electron, TransmissionPolymerase Chain ReactionRNA, Messenger
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