Pharmaceutical biology · 2026
The effect of provenance and species on the chemical composition of Epilobium herbal tea.
СтатьяСравнительное исследованиеНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Mykhailenko Olha, Jalil Banaz, Uminska Kateryna, Ivanauskas Liudas, Gudžinskas Zigmantas, Heinrich Michael
Аннотация
BACKGROUND: Commercial herbal teas marketed as Epilobium herb are widely consumed in Europe for prostate-related and anti-inflammatory effects. However, they are often sold under generic names without clear species differentiation or quality specifications, raising concerns about chemical consistency and therapeutic equivalence.
OBJECTIVE: To evaluate the chemical equivalence of commercial Epilobium products and refine the framework for quality marker selection by integrating pharmacological relevance with structural assessment.
MATERIALS AND METHODS: Sixteen commercial tea samples labeled as E. angustifolium, E. parviflorum, E. hirsutum, from eight European countries were analyzed using high-performance thin-layer chromatography (HPTLC) and validated by HPLC-DAD. Candidate markers were prioritized using the Herbal Chemical Marker Ranking System (Herb MaRS) and further assessed via Pan-Assay Interference Compounds (PAINS) structural filters.
RESULTS: Significant qualitative and quantitative heterogeneity was observed across all samples, including those labeled as the same species. Oenothein B was the dominant compound but exhibited high variability (7.9-48.7 mg/g DW; CV >50%) and was significantly higher in E. hirsutum (p < 0.05). Flavonol glycosides (e.g., hyperoside, isoquercitrin) also varied substantially. Although species identity influenced phenolic profiles, overlapping concentration ranges indicated a lack of chemical equivalence among products. Geographic origin contributed additional but secondary variability.
DISCUSSION: Oenothein B, hyperoside, and isoquercitrin were identified as priority quality markers based on abundance and analytical suitability. Structural reassessment demonstrated that pharmacological ranking alone may be misleading for polyphenol-rich matrices due to redox-related interference.
CONCLUSION: Commercial Epilobium teas differ chemically and are not interchangeable. A structure-informed approach is essential for reliable quality assessment and for aligning commercial products with pharmacopeial standards.
OBJECTIVE: To evaluate the chemical equivalence of commercial Epilobium products and refine the framework for quality marker selection by integrating pharmacological relevance with structural assessment.
MATERIALS AND METHODS: Sixteen commercial tea samples labeled as E. angustifolium, E. parviflorum, E. hirsutum, from eight European countries were analyzed using high-performance thin-layer chromatography (HPTLC) and validated by HPLC-DAD. Candidate markers were prioritized using the Herbal Chemical Marker Ranking System (Herb MaRS) and further assessed via Pan-Assay Interference Compounds (PAINS) structural filters.
RESULTS: Significant qualitative and quantitative heterogeneity was observed across all samples, including those labeled as the same species. Oenothein B was the dominant compound but exhibited high variability (7.9-48.7 mg/g DW; CV >50%) and was significantly higher in E. hirsutum (p < 0.05). Flavonol glycosides (e.g., hyperoside, isoquercitrin) also varied substantially. Although species identity influenced phenolic profiles, overlapping concentration ranges indicated a lack of chemical equivalence among products. Geographic origin contributed additional but secondary variability.
DISCUSSION: Oenothein B, hyperoside, and isoquercitrin were identified as priority quality markers based on abundance and analytical suitability. Structural reassessment demonstrated that pharmacological ranking alone may be misleading for polyphenol-rich matrices due to redox-related interference.
CONCLUSION: Commercial Epilobium teas differ chemically and are not interchangeable. A structure-informed approach is essential for reliable quality assessment and for aligning commercial products with pharmacopeial standards.