Journal of the science of food and agriculture · 2025
Methionine affects the freeze-drying resistance of Lactiplantibacillus plantarum LIP-1 by improving its antioxidant capacity.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zheng Yijian, Chen Zichao, Wang Ruixue, Yang Ying, Yang Youxin, E Jingjing, Wang Junguo
Аннотация
BACKGROUND: Lactic acid bacteria is an essential industrial strain, and improving its freeze-drying survival rate is the key challenge to ensuring the activity and stability of bacterial powder. Although medium optimization has been shown to strengthen strain freeze-drying tolerance, the mechanism by which amino acids repair freeze-drying damage in lactic acid bacteria remains unclear. This study investigated the effects of methionine on the freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 and explored the underlying protective mechanisms.
RESULTS: The study demonstrates that supplementing the medium with 0.06 g/L methionine significantly improved the freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 (P < 0.05). Further analysis revealed that the strain significantly reduced intracellular reactive oxygen species levels through metabolizing methionine (P < 0.05), decreased the oxidation degree of unsaturated fatty acids in the cell membrane, and reduced cell membrane damage, thereby strengthening the freeze-drying resistance of the strain.
CONCLUSION: Methionine can enhance the freeze-drying resistance of Lactiplantibacillus plantarum LIP-1 by enhancing antioxidant capacity and maintaining the stability of the subcellular structure. This study provides a specific reference value for improving the freeze-drying survival rate of lactic acid bacteria by modifying the medium conditions. © 2025 Society of Chemical Industry.
RESULTS: The study demonstrates that supplementing the medium with 0.06 g/L methionine significantly improved the freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 (P < 0.05). Further analysis revealed that the strain significantly reduced intracellular reactive oxygen species levels through metabolizing methionine (P < 0.05), decreased the oxidation degree of unsaturated fatty acids in the cell membrane, and reduced cell membrane damage, thereby strengthening the freeze-drying resistance of the strain.
CONCLUSION: Methionine can enhance the freeze-drying resistance of Lactiplantibacillus plantarum LIP-1 by enhancing antioxidant capacity and maintaining the stability of the subcellular structure. This study provides a specific reference value for improving the freeze-drying survival rate of lactic acid bacteria by modifying the medium conditions. © 2025 Society of Chemical Industry.