European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021
Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples.
СтатьяНаблюдательное исследованиеНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Kaku Norihito, Hashiguchi Kohji, Akamatsu Norihiko, Wakigawa Fumiko, Matsuda Junichi, Komaru Kenzo, Nakao Takumi, Harada Yosuke, Hara Atsuko, Uno Naoki, Sakamoto Kei, Morinaga Yoshitomo, Kitazaki Takeshi, Hasegawa Hiroo, Miyazaki Taiga, Fukuda Masaaki, Izumikawa Koichi, Mukae Hiroshi, Yanagihara Katsunori
Аннотация
We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.