Saliva is a reliable and accessible source for the detection of SARS-CoV-2.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Herrera Luis A, Hidalgo-Miranda Alfredo, Reynoso-Noverón Nancy, Meneses-García Abelardo A, Mendoza-Vargas Alfredo, Reyes-Grajeda Juan P, Vadillo-Ortega Felipe, Cedro-Tanda Alberto, Peñaloza Fernando, Frías-Jimenez Emmanuel, Arriaga-Canon Cristian, Ruiz Rosaura, Angulo Ofelia, López-Villaseñor Imelda, Amador-Bedolla Carlos, Vilar-Compte Diana, Cornejo Patricia, Cisneros-Villanueva Mireya, Hurtado-Cordova Eduardo, Cendejas-Orozco Mariana, Hernández-Morales José S, Moreno Bernardo, Hernández-Cruz Irwin A, Herrera César A, García Francisco, González-Woge Miguel A, Munguía-Garza Paulina, Luna-Maldonado Fernando, Sánchez-Vizcarra Antonia, Osnaya Vincent G, Medina-Molotla Nelly, Alfaro-Mora Yair, Cáceres-Gutiérrez Rodrigo E, Tolentino-García Laura, Rosas-Escobar Patricia, Román-González Sergio A, Escobar-Arrazola Marco A, Canseco-Méndez Julio C, Ortiz-Soriano Diana R, Domínguez-Ortiz Julieta, González-Barrera Ana D, Aparicio-Bautista Diana I, Cruz-Rangel Armando, Alarcón-Zendejas Ana Paula, Contreras-Espinosa Laura, González Rodrigo, Guerra-Calderas Lissania, Meraz-Rodríguez Marco A, Montalvo-Casimiro Michel, Montiel-Manríquez Rogelio, Torres-Arciga Karla, Venegas Daniela, Juárez-González Vasti, Guajardo-Barreto Xiadani, Monroy-Martínez Verónica, Guillén Daniel, Fernández Jacquelina, Herrera Juliana, León-Rodriguez Renato, Canela-Pérez Israel, Ruíz-Ordaz Blanca H, Valdez-Vazquez Rafael, Bertin-Montoya Jennifer, Niembro-Ortega María, Villegas-Acosta Liudmila, López-Castillo Daniela, Soriano-Ríos Andrea, Gastelum-Ramos Michael, Zamora-Barandas Tonatiuh, Morales-Baez Jorge, García-Rodríguez María, García-Martínez Mariano, Nieto-Patlán Erik, Quirasco-Baruch Maricarmen, López-Martínez Irma, Ramírez-Gonzalez Ernesto, Olivera-Díaz Hiram, Escobar-Escamilla Noe
Аннотация
METHODS: A total of 2107 paired samples were collected from asymptomatic healthcare and office workers in Mexico City. Sixty of these samples were also analyzed in two other independent laboratories for concordance analysis. Sample processing and analysis of virus genetic material were performed according to standard protocols described elsewhere. A pooling analysis was performed by analyzing the saliva pool and the individual pool components.
RESULTS: The concordance between NPS and saliva results was 95.2% (kappa 0.727, p = 0.0001) and 97.9% without considering inconclusive results (kappa 0.852, p = 0.0001). Saliva had a lower number of inconclusive results than NPS (0.9% vs 1.9%). Furthermore, saliva showed a significantly higher concentration of both total RNA and viral copies than NPS. Comparison of our results with those of the other two laboratories showed 100% and 97% concordance. Saliva samples are stable without the use of any preservative, and a positive SARS-CoV-2 sample can be detected 5, 10, and 15 days after collection when the sample is stored at 4 °C.
CONCLUSIONS: The study results indicate that saliva is as effective as NPS for the identification of SARS-CoV-2-infected asymptomatic patients. Sample pooling facilitates the analysis of a larger number of samples, with the benefit of cost reduction.