Bratislavske lekarske listy · 2015
Effects of Nigella sativa on heat-induced testis damage in mouse.
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Mohajeri D, Kaffashi Elahi R
Аннотация
BACKGROUND: Infertility is one of the major health problems.
OBJECTIVES: The aim of this study was to evaluate the effects of Nigella sativa on heat-induced testicular damage.
METHODS: Forty male mice were randomly divided into the four equal groups as Control, Heat stressed and, Heated and treated with Nigella sativa 10 % and 20 % in diet. The scrotum of mice except to the control mice were immersed for 15 min in a water bath at 43 °C. Animals in the control group were treated identically except that the water bath was maintained at 23 °C. Fifty days after the heating, blood samples were collected for testosterone levels. Testes were removed for the measurement of seminiferous tubules diameter and percentage of spermatogenesis and oxidant/antioxidant status.
RESULTS: Heating stress significantly reduced blood testosterone level and increased lipid peroxidation product and decreased antioxidant activities of superoxide dismutase, catalase and glutathione peroxidase (p < 0.01). Nigella sativa treatment significantly increased blood testosterone level and decreased testis malondialdehyde level and increased antioxidant enzymes activities (p < 0.05). In the mice treated with Nigella sativa, testes illustrated normal spermatogenesis and structure.
CONCLUSION: The results indicated that supplementation of Nigella sativa in diet improves spermatogenesis and antioxidant status after a short exposure of the mouse testis to heat (Tab. 1, Fig. 4, Ref. 45).
OBJECTIVES: The aim of this study was to evaluate the effects of Nigella sativa on heat-induced testicular damage.
METHODS: Forty male mice were randomly divided into the four equal groups as Control, Heat stressed and, Heated and treated with Nigella sativa 10 % and 20 % in diet. The scrotum of mice except to the control mice were immersed for 15 min in a water bath at 43 °C. Animals in the control group were treated identically except that the water bath was maintained at 23 °C. Fifty days after the heating, blood samples were collected for testosterone levels. Testes were removed for the measurement of seminiferous tubules diameter and percentage of spermatogenesis and oxidant/antioxidant status.
RESULTS: Heating stress significantly reduced blood testosterone level and increased lipid peroxidation product and decreased antioxidant activities of superoxide dismutase, catalase and glutathione peroxidase (p < 0.01). Nigella sativa treatment significantly increased blood testosterone level and decreased testis malondialdehyde level and increased antioxidant enzymes activities (p < 0.05). In the mice treated with Nigella sativa, testes illustrated normal spermatogenesis and structure.
CONCLUSION: The results indicated that supplementation of Nigella sativa in diet improves spermatogenesis and antioxidant status after a short exposure of the mouse testis to heat (Tab. 1, Fig. 4, Ref. 45).