Clinical biochemistry · 2014
Validation of interleukin 28B genotyping assay for clinical use.
СтатьяВалидационное исследованиеНазвание и аннотация: Перевод пока не подготовлен
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Cervinski Mark A, Sam Soya S, Steinmetz Heather B, Wood Brendan, Tsongalis Gregory J
Аннотация
OBJECTIVES: The favorable CC genotype at rs12979860 upstream of the interleukin (IL)-28B gene is correlated with a greater post-treatment sustained virologic response rate in chronic hepatitis C infected patients. We report on our validation of a clinical genotyping assay for rs12979860 polymorphisms in the IL28B locus.
DESIGN AND METHODS: The rs12979860 genotype was determined using a TaqMan® Real-Time PCR allelic discrimination assay with primers and probes specific for the C and T alleles on the Applied Biosystems 7500 Fast Real-Time PCR System.
RESULTS: The rs12979860 genotype determined by our assay was concordant with the genotypes obtained from a reference laboratory. The allelic frequency was similar to that reported in the HapMap project (rs12979860 C=0.65, T=0.35) and did not deviate from Hardy-Weinberg equilibrium.
CONCLUSION: Clinical availability of this assay in conjunction with other factors will allow the prediction of the individual patient's response to therapy.
DESIGN AND METHODS: The rs12979860 genotype was determined using a TaqMan® Real-Time PCR allelic discrimination assay with primers and probes specific for the C and T alleles on the Applied Biosystems 7500 Fast Real-Time PCR System.
RESULTS: The rs12979860 genotype determined by our assay was concordant with the genotypes obtained from a reference laboratory. The allelic frequency was similar to that reported in the HapMap project (rs12979860 C=0.65, T=0.35) and did not deviate from Hardy-Weinberg equilibrium.
CONCLUSION: Clinical availability of this assay in conjunction with other factors will allow the prediction of the individual patient's response to therapy.