Current eye research · 2007
Molecular analysis of neurolysin expression in the rat and bovine ciliary body.
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Bertazolli-Filho Rubens, Coca-Prados Miguel, Haddad Antonio, Laicine Eduardo Miguel
Аннотация
PURPOSE: This paper deals with the capability of the ciliary epithelium to express neurolysin, involved in the inactivation of numerous neuropeptides.
METHODS: Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization.
RESULTS: The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium.
CONCLUSIONS: These results indicate that the ciliary epithelium expresses neurolysin.
METHODS: Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization.
RESULTS: The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium.
CONCLUSIONS: These results indicate that the ciliary epithelium expresses neurolysin.