Bioactive materials · 2026
Development of a PTEN-siRNA activated scaffold to promote axonal regrowth following spinal cord injury.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
McGuire Tara K, Stasiewicz Martyna, O'Connor Cian, Woods Ian, Maughan Jack, Dobricic Marko, Brunetti Giulio, Dixon James E, Dervan Adrian G, O'Brien Fergal J
Аннотация
This study showcases the successful development of a PTEN-siRNA activated scaffold for SCI repair applications. Initially, siRNA was combined with the novel non-viral glycosaminoglycan-binding enhanced transduction (GET) peptide vector (image of GET vector from [22]). The formulated GET-siRNA nanoparticles effectively transfected primary neurons, a cell type generally considered challenging to transfect. Subsequently, siRNA-nanoparticles were incorporated into a hyaluronic acid scaffold enriched with neurotrophic extracellular matrix proteins (collagen IV and fibronectin) developed by our laboratory for spinal cord repair applications. Functionalisation of these scaffolds with PTEN-siRNA nanoparticles significantly enhanced their capacity to promote neurite outgrowth.Image 1.