[Cationic Blue O staining for the cytological detection of Helicobacter pylori].

This investigation was undertaken to study whether cationic blue O staining might be used to improve the cytological diagnosis of H. pylori infection at gastric and extragastric sites. One hundred and sixty-four patients with chronic inflammatory diseases of the stomach and gallbladder were examined with cytological and molecular studies. Impression smears from the gastric mucosa and bile portions A, B, and C were studied. The stain proposed by the authors ensures a high efficiency in detecting H. pylori in different biological materials (gastric biopsy specimens, bile) with the determination of the degree of contamination makes it possible to evaluate the morphological changes in the gastric mucosa and bile epithelium. The detection results of smear H. pylori correlated with those of DNA in this microorganism, by using the polymerase chain reaction. The proposed cytological study may be recommended for both the primary diagnosis of H. pylori infection in patients with diseases of the stomach, duodenum, and gallbladder and the monitoring eradication therapy and its deserves extensive use by cytological laboratories.