Anti-Toxoplasma Activity of Copper Nanoparticles (CuNPs) Synthesized Using Conocarpus erectus L.: An In Vitro and In Vivo Study.

BACKGROUND

This study evaluates in vitro and in vivo anti-Toxoplasma effects of copper nanoparticles (CuNPs) green synthesized using Conocarpus erectus L.

MATERIALS AND

METHODS

CuNPs were green synthesized using the aqueous extract of Conocarpus erectus L. An MTT assay was performed on Hella cells to evaluate the cell viability. The in vitro anti-Toxoplasma activity of various concentrations of CuNPs (20-160 ppm) for 1, 2, 4, and 8 h at room temperature was assessed against the Toxoplasma gondii RH tachyzoites. Moreover, the flow cytometry test was carried out to confirm the results. For in vivo assessment, BALB/c mice were infected with RH strains, subsequently treated with CuNPs, and compared with the control group.

RESULTS

CuNPs had a particle size of less than 20 nm, with a maximum peak at 315 nm, according to transmission electron microscopy. The highest mortality rate was observed at 160 ppm concentration, and after 8 h of exposure, it was demonstrated by the result of flow cytometry. Furthermore, oral administration of CuNPs increased oral bioavailability and mice survival time and reduced parasitemia. Green synthesized CuNPs by Conocarpus erectus L. reduced the tachyzoites of Toxoplasma gondii, RH strain in vitro, and increased the survival time of infected mice treated with CuNPs compared to the control group.

CONCLUSIONS

To achieve effective treatment against toxoplasmosis, it is suggested that more studies will be done on other strains, especially type II. Finally, it seems that the use of CuNPs can be helpful as a supplementary treatment alongside common treatments.