A highly sensitive fluorogenic assay for trypsin in urine: a simple diagnostic approach for pancreatic diseases.

A water-soluble pyrene-malonate (PyM) probe enables ultrasensitive, non-invasive detection of urinary trypsin for early pancreatic disease screening. Protamine binding induces aggregation and excimer formation, while trypsin-mediated cleavage disassembles the aggregates and enables monomer fluorescence recovery. The system reliably detects trypsin in 10% human urine within physiological levels.