The Effect of Probe Pressure on In Vivo Single Fiber Reflectance Spectroscopy.

Introduction: Single fiber reflectance spectroscopy (SFRS) is a noninvasive procedure to quantitate tissue absorption and scattering properties. It can be used to diagnose different diseases such as malignancy and pre-cancerous conditions. The measurement is done with a fiber optic probe in contact with the tissue surface. Herein, the effect of probe pressure on the extracted parameters from human lip spectra was studied. Methods: Thirty-three normal subjects were examined with three exerted pressure levels on the right, middle and left parts of their lips. Results: The results showed variation of spectroscopic parameters with different pressure levels. However, the effect was seen between a very mild contact (pressure 1) and the other reasonably practical pressure levels normally used in the medical centers. Conclusion: SFRS can be used as a reliable diagnostic tool in clinics.