Clinical utility of plasma microbial cell-free DNA sequencing for early diagnosis of toxoplasmosis in high-risk patients: a five-patient case series.

From 2017 to 2025, we reviewed plasma microbial cell-free DNA sequencing (mcfDNA NGS) results detecting Toxoplasma gondii. Five patients were identified. Median turnaround was 3 days (2-5). mcfDNA initiated therapy in two and supported empiric treatment in three but required clinical context for interpretation.